Journal of Sleep Sciences 0. 0(0):.

Acute Sleep Deprivation Decreases Anxiety Behavior via GABA-A Receptor Activation in Central Nucleus of Amygdala
Hoda Parsa, Alireza Imani, Mahdieh Faghihi


Background and Objective: Amygdala contains central nucleus which is the region rich of GABA-A receptors and plays a critical role in modulating behavior and sleep homeostasis. Also,dysfunction of amygdala probably contributes to anxiety and mood disorders. In this study, we evaluated the association between acute sleep deprivation and anxiety through GABA-A receptor in central nucleus of amygdala.

Materials and Methods: Thirty five male rats were bilaterally cannulated in central nucleus of amygdala (CeA), randomly divided into four groups (n=7); control (CON), acute sleep deprivation (ASD), bicuculline (BIC) (as a GABA-A receptor blocker), bicuculline + acute sleep deprivation (BIC +ASD). Saline was injected Intra-CeA for the first three groups and others received Intra-CeA bicuculline (0.1 nmol/0.5μl in volume of 0.5 μl same at each side).

Results: Intra-CeA injection of bicuculline increased the level of anxiety compared to control group. Induction of 24h acute sleep deprivation immediately after bicuculline injection, led to decrease in the anxiety level when compared to bicuculline group, and we found no statistically difference between control and ASD groups.

Conclusion: Intra-CeA injection of bicuculline increased anxiety level while induction of acute sleep deprivation decreased bicuculline-induced anxiety.


Sleep deprivation, Amygdala, Anxiety, GABA

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