Tehran University of Medical Sciences Journal of Sleep Sciences 2476-2938 1 4 2017 04 09 139 142 EN Hoda Parsa Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mahdieh Faghihi Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Alireza Imani Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2016 06 29 2016 08 27 Background and Objective: Amygdala contains central nucleus which is the region rich of γ-aminobutyric acid (GABA-A) receptors and plays a key role in modulating behavior and sleep homeostasis. Furthermore, dysfunction of amygdala probably contributes to anxiety and mood disorders. In this study, we evaluated the association between acute sleep deprivation (ASD) and anxiety through GABA-A receptor in the central nucleus of the amygdala (CeA). Materials and Methods: A total of 35 male rats were bilaterally cannulated in CeA, randomly divided into four groups (n = 7); control (CON), ASD, bicuculline (BIC) (as a GABA-A receptor blocker), BIC + ASD. Saline was in-jected intra-CeA for the first three groups and others received intra-CeA BIC (0.1 nmol/0.5 μl in volume of 0.5 μl same at each side). Results: Intra-CeA injection of BIC increased the level of anxiety compared to control group. Induction of 24 hours ASD immediately after BIC injection, led to decrease in the anxiety level when compared to BIC group, and we found no statistically difference between control and ASD groups. Conclusion: Intra-CeA injection of BIC increased anxiety level while induction of ASD decreased BIC-induced anxiety. https://jss.tums.ac.ir/index.php/jss/article/view/33 https://jss.tums.ac.ir/index.php/jss/article/download/33/49