Effect of Sleep Restriction on Cardiometabolic and Haemoinflammatory Parameters in Adult Male Wistar Rats
,
Abstract
Background and Objective: While insufficient sleep remains an under-recognized public health issue across the globe, there is paucity and heterogeneity of data regarding its cardiometabolic and haemoinflammatory implications. We, therefore, aimed to evaluate the impact of chronic sleep restriction on cardiometabolic and haemoinflammatory parame-ters in rats.
Materials and Methods: 16 male Wistar rats (aged 8-10 weeks) were randomly assigned into equal control or sleep restriction groups. Gentle handling was used to induce sleep restriction for six weeks. Fasting weight and blood sugar were obtained and lipids were analyzed using their respective Randox kits. Malondialdehyde (MDA) levels and catalase (CAT) and superoxide dismutase (SOD) activities were assayed. Full blood count and CD4+ T cell count were deter-mined using automated analyzer. Data were analyzed using Student’s t-test, with level of significance set at P ≤ 0.05, via SPSS software.
Results: Chronic sleep restriction caused significant initial weight loss, increase in feed consumption, and percentage in-crease in fasting blood sugar (FBS) (32% vs. 15%). We also noted the triglyceride-glucose (TyG) index of sleep-restricted rats to be significantly higher (6.22) than that of controls (5.62). In addition, a significant reduction in monocyte count, monocyte-lymphocyte ratio (MLR), and absolute CD4+ cell count among the sleep-restricted rats was observed.
Conclusion: Our findings have provided objective evidence that, over the course of 6 weeks, 5 hours of sleep re-striction had caused body weight gain, hyperglycaemia, insulin resistance, and impairment in immunoinflammatory status; hence, it could be a risk factor for developing cardiometabolic syndrome and immune-related disorders.
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15. De Lorenzo BHP, Novaes E Brito RR, Paslar LT, et al. Chronic sleep restriction impairs the antitumor immune response in mice. Neuroimmunomodulation 2018; 25: 59-67.
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17. Du T, Yuan G, Zhang M, et al. Clinical usefulness of lipid ratios, visceral adiposity indicators, and the triglycerides and glucose index as risk markers of insul in resistance. Cardiovasc Diabetol 2014; 13: 146.
18. Everson CA, Wehr TA. Nutritional and metabolic adaptations to prolonged sleep deprivation in the rat. Am J Physiol 1993; 264: R376-R387.
19. Fridovich I. Superoxide dismutases. An adaptation to a paramagnetic gas. J Biol Chem 1989; 264: 7761-4.
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24. Ho JM, Ducich NH, Nguyen NK, et al. Acute sleep disruption- and high-fat diet-induced hypothalamic in-flammation are not related to glucose tolerance in mice. Neurobiol Sleep Circadian Rhythms 2018; 4: 1-9.
25. Ikewuchi J, Ikewuchi C. Alteration of plasma lipid profiles and Atherogenic indices by Stachytarpheta jamaicensis L. (Vahl). Biokemistri 2009; 21: 71-7.
26. Kim D, Hoyos CM, Mokhlesi B, et al. Editorial: Metabolic health in normal and abnormal sleep. Front Endocrinol (Lausanne) 2020; 11: 131.
27. Korostovtseva L, Alieva A, Rotar O, et al. sleep duration, lipid profile and insulin resistance: Potential role of lipoprotein(a). Int J Mol Sci 2020; 21: e131487.
28. Kuula L, Pesonen AK, Kajantie E, et al. Sleep and lipid profile during transition from childhood to adolescence. J Pediatr 2016; 177: 173-8.
29. Said EA, Al-Abri MA, Al-Saidi I, et al. Sleep deprivation alters neutrophil functions and levels of Th1-related chemokines and CD4(+) T cells in the blood. Sleep Breath 2019; 23: 1331-9.
30. Mullington JM, Haack M, Toth M, et al. Cardiovascular, inflammatory, and metabolic consequences of sleep deprivation. Prog Cardiovasc Dis 2009; 51: 294-302.
31. Nakao T, Yasumoto A, Tokuoka S, et al. The impact of night-shift work on platelet function in healthy medical staff. J Occup Health 2018; 60: 324-32.
32. Patel SR, Malhotra A, Gao X, et al. A prospective study of sleep duration and pneumonia risk in women. Sleep 2012; 35: 97-101.
33. Prather AA, Hall M, Fury JM, et al. Sleep and antibody response to hepatitis B vaccination. Sleep 2012; 35: 1063-9.
34. Prather AA, Janicki-Deverts D, Hall MH, et al. Behaviorally assessed sleep and susceptibility to the common cold. Sleep 2015; 38: 1353-9.
35. Roberson PA. The effect of sleep restriction on coagulation and fibrinolysis after heavy exercise [MSc Thesis]. Harrisonburg, VA: James Madison University; 2021.
36. McEwen BS, Karatsoreos IN. Sleep deprivation and circadian disruption: stress, allostasis, and allostatic load. Sleep Med Clin 2015; 10: 1-10.
37. Sargent C, Zhou X, Matthews RW, et al. Daily rhythms of hunger and satiety in healthy men during one week of sleep restriction and circadian misalignment. Int J Environ Res Public Health 2016; 13: 170.
38. Son SM, Park EJ, Cho YH, et al. Association between weekend catch-up sleep and metabolic syndrome with sleep restriction in korean adults: a cross-sectional study using KNHANES. Diabetes Metab Syndr Obes 2020; 13: 1465-71.
39. Spaeth AM, Dinges DF, Goel N. Resting metabolic rate varies by race and by sleep duration. Obesity (Silver Spring) 2015; 23: 2349-56.
40. Villafuerte G, Miguel-Puga A, Rodriguez EM, et al. Sleep deprivation and oxidative stress in animal models: A systematic review. Oxid Med Cell Longev 2015; 2015: 234952.
41. Visniauskas B, Pedro AN, Dias CC, et al. The impact of sleep deprivation in the bone marrow progenitors and the role of angiotensin-converting enzyme. Sleep 2019; 42: A89.
42. Zou X, Huang W, Lu F, et al. The effects of Jiao-Tai-Wan on sleep, inflammation and insulin resistance in obesity-resistant rats with chronic partial sleep deprivation. BMC Complement Altern Med 2017; 17: 165.
2. Aebi H. Catalase. In Bergmeyer HV, editor. Methods in enzymatic analysis. New York, NY: Academic Press Inc.; 1974. p. 673-86.
3. Akboga MK, Yayla C, Balci KG, et al. Relationship between serum albumin level and monocyte-to-high-130 J Sleep Sci,
density lipoprotein cholesterol ratio with saphenous vein graft disease in coronary bypass. Thorac Cardiovasc Surg 2017; 65: 315-21.
4. Albro PW, Corbett JT, Schroeder JL. Application of the thiobarbiturate assay to the measurement of lipid peroxidation products in microsomes. J Biochem Bio phys Methods 1986; 13: 185-94.
5. Archer SN, Oster H. How sleep and wakefulness influence circadian rhythmicity: effects of insufficient and mistimed sleep on the animal and human transcriptome. J Sleep Res 2015; 24: 476-93.
6. Bodosi B, Gardi J, Hajdu I, et al. Rhythms of ghrelin, leptin, and sleep in rats: effects of the normal diurnal cycle, restricted feeding, and sleep deprivation. Am J Physiol Regul Integr Comp Physiol 2004; 287: R1071-R1079.
7. Buxton OM, Cain SW, O'Connor SP, et al. Adverse metabolic consequences in humans of prolonged sleep restriction combined with circadian disruption. Sci Transl Med 2012; 4: 129ra43.
8. Castelli WP, Abbott RD, McNamara PM. Summary estimates of cholesterol used to predict coronary heart disease. Circulation 1983; 67: 730-4.
9. Chattu VK, Manzar MD, Kumary S, et al. The Global Problem of Insufficient Sleep and Its Serious Public Health Implications. Healthcare (Basel) 2018; 7.
10. Chattu VK, Sakhamuri SM, Kumar R, et al. Insufficient Sleep Syndrome: Is it time to classify it as a major noncommunicable disease? Sleep Sci 2018; 11: 56-64.
11. Christoffersson G, Vagesjo E, Pettersson US, et al. Acute sleep deprivation in healthy young men: impact on population diversity and function of circulating neutrophils. Brain Behav Immun 2014; 41: 162-72.
12. Cirelli C, Benca R, Eichler AF. Insufficient Sleep: Definition, Epidemiology, and Adverse Outcomes [Online]. [cited 2019 Dec 16]; Available from: URL: https://www.uptodate.com/contents/insufficient-sleep-definition-epidemiology-and-adverse-outcomes
13. Curtis DS, Fuller-Rowell TE, El-Sheikh M, et al. Habitual sleep as a contributor to racial differences in cardiometabolic risk. Proc Natl Acad Sci USA 2017; 114: 8889-94.
14. Das BS, Thurnham DI, Patnaik JK, et al. Increased plasma lipid peroxidation in riboflavin-deficient, malaria-infected children. Am J Clin Nutr 1990; 51: 859-63.
15. De Lorenzo BHP, Novaes E Brito RR, Paslar LT, et al. Chronic sleep restriction impairs the antitumor immune response in mice. Neuroimmunomodulation 2018; 25: 59-67.
16. Dissi GM, Ibrahim SA, Tanko Y, et al. Models of modern-day circadian rhythm disruption and their diabetogenic potentials in adult male Wistar rats. Saudi J Health Sci 2020. [In Press].
17. Du T, Yuan G, Zhang M, et al. Clinical usefulness of lipid ratios, visceral adiposity indicators, and the triglycerides and glucose index as risk markers of insul in resistance. Cardiovasc Diabetol 2014; 13: 146.
18. Everson CA, Wehr TA. Nutritional and metabolic adaptations to prolonged sleep deprivation in the rat. Am J Physiol 1993; 264: R376-R387.
19. Fridovich I. Superoxide dismutases. An adaptation to a paramagnetic gas. J Biol Chem 1989; 264: 7761-4.
20. Gao E, Hou J, Zhou Y, et al. Mediation effect of platelet indices on the association of daytime nap duration with 10-year ASCVD risk. Platelets 2021; 32: 82-9.
21. Hafner M, Stepanek M, Taylor J, et al. Why sleep matters-the economic costs of insufficient sleep: a cross-country comparative analysis. Rand Health Q 2017; 6: 11.
22. Hammouda O, Romdhani M, Chaabouni Y, et al. Diurnal napping after partial sleep deprivation affected hematological and biochemical responses during repeated sprint. Biol Rhythm Res 2018; 49: 927-39.
23. Haspel JA, Anafi R, Brown MK, et al. Perfect timing: circadian rhythms, sleep, and immunity - an NIH workshop summary. JCI Insight 2020; 5: e131487.
24. Ho JM, Ducich NH, Nguyen NK, et al. Acute sleep disruption- and high-fat diet-induced hypothalamic in-flammation are not related to glucose tolerance in mice. Neurobiol Sleep Circadian Rhythms 2018; 4: 1-9.
25. Ikewuchi J, Ikewuchi C. Alteration of plasma lipid profiles and Atherogenic indices by Stachytarpheta jamaicensis L. (Vahl). Biokemistri 2009; 21: 71-7.
26. Kim D, Hoyos CM, Mokhlesi B, et al. Editorial: Metabolic health in normal and abnormal sleep. Front Endocrinol (Lausanne) 2020; 11: 131.
27. Korostovtseva L, Alieva A, Rotar O, et al. sleep duration, lipid profile and insulin resistance: Potential role of lipoprotein(a). Int J Mol Sci 2020; 21: e131487.
28. Kuula L, Pesonen AK, Kajantie E, et al. Sleep and lipid profile during transition from childhood to adolescence. J Pediatr 2016; 177: 173-8.
29. Said EA, Al-Abri MA, Al-Saidi I, et al. Sleep deprivation alters neutrophil functions and levels of Th1-related chemokines and CD4(+) T cells in the blood. Sleep Breath 2019; 23: 1331-9.
30. Mullington JM, Haack M, Toth M, et al. Cardiovascular, inflammatory, and metabolic consequences of sleep deprivation. Prog Cardiovasc Dis 2009; 51: 294-302.
31. Nakao T, Yasumoto A, Tokuoka S, et al. The impact of night-shift work on platelet function in healthy medical staff. J Occup Health 2018; 60: 324-32.
32. Patel SR, Malhotra A, Gao X, et al. A prospective study of sleep duration and pneumonia risk in women. Sleep 2012; 35: 97-101.
33. Prather AA, Hall M, Fury JM, et al. Sleep and antibody response to hepatitis B vaccination. Sleep 2012; 35: 1063-9.
34. Prather AA, Janicki-Deverts D, Hall MH, et al. Behaviorally assessed sleep and susceptibility to the common cold. Sleep 2015; 38: 1353-9.
35. Roberson PA. The effect of sleep restriction on coagulation and fibrinolysis after heavy exercise [MSc Thesis]. Harrisonburg, VA: James Madison University; 2021.
36. McEwen BS, Karatsoreos IN. Sleep deprivation and circadian disruption: stress, allostasis, and allostatic load. Sleep Med Clin 2015; 10: 1-10.
37. Sargent C, Zhou X, Matthews RW, et al. Daily rhythms of hunger and satiety in healthy men during one week of sleep restriction and circadian misalignment. Int J Environ Res Public Health 2016; 13: 170.
38. Son SM, Park EJ, Cho YH, et al. Association between weekend catch-up sleep and metabolic syndrome with sleep restriction in korean adults: a cross-sectional study using KNHANES. Diabetes Metab Syndr Obes 2020; 13: 1465-71.
39. Spaeth AM, Dinges DF, Goel N. Resting metabolic rate varies by race and by sleep duration. Obesity (Silver Spring) 2015; 23: 2349-56.
40. Villafuerte G, Miguel-Puga A, Rodriguez EM, et al. Sleep deprivation and oxidative stress in animal models: A systematic review. Oxid Med Cell Longev 2015; 2015: 234952.
41. Visniauskas B, Pedro AN, Dias CC, et al. The impact of sleep deprivation in the bone marrow progenitors and the role of angiotensin-converting enzyme. Sleep 2019; 42: A89.
42. Zou X, Huang W, Lu F, et al. The effects of Jiao-Tai-Wan on sleep, inflammation and insulin resistance in obesity-resistant rats with chronic partial sleep deprivation. BMC Complement Altern Med 2017; 17: 165.
| Files | ||
| Issue | Vol 5 No 4 (2020): Autumn | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/jss.v5i4.7804 | |
| Keywords | ||
| Sleep deprivation Metabolic syndrome Blood cell count CD4 positive cells | ||
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How to Cite
1.
Gambo M, Salisu AI, Tanko Y, Aliyu M. Effect of Sleep Restriction on Cardiometabolic and Haemoinflammatory Parameters in Adult Male Wistar Rats. J Sleep Sci. 2021;5(4):124-131.
